Evaluation of the antifungal effect and adhesive strength of a denture adhesive supplemented with nystatin

Autores

  • Graziele Cristina Alvim Universidade de Uberaba, Departamento de Biomateriais. Uberaba, Minas Gerais, Brasil
  • Camilla Beatriz da Silva Universidade de Uberaba, Departamento de Biomateriais. Uberaba, Minas Gerais, Brasil
  • Viviane de Cássia Oliveira Faculdade de Odontologia de Ribeirão Preto, Universidade de São Paulo, Departamento de Materiais Dentários e Prótese. Ribeirão Preto, São Paulo, Brasil
  • Andréa Cândido dos Reis Faculdade de Odontologia de Ribeirão Preto, Universidade de São Paulo, Departamento de Materiais Dentários e Prótese. Ribeirão Preto, São Paulo, Brasil
  • Evandro Watanabe Faculdade de Odontologia de Ribeirão Preto, Universidade de São Paulo,Departamento de Odontologia Restauradora. Ribeirão Preto, São Paulo, Brasil
  • César Penazzo Lepri Universidade de Uberaba, Departamento de Biomateriais. Uberaba, Minas Gerais, Brasil
  • Denise Tornavoi de Castro Universidade de Uberaba, Departamento de Biomateriais. Uberaba, Minas Gerais, Brasil

DOI:

https://doi.org/10.26694/repis.v9i1.4337

Palavras-chave:

adhesive, Candida albicans, dental prosthesis, nystatin

Resumo

Introduction: The scientific basis related to the effect of incorporating antifungals on the properties of denture adhesives is scarce. This study incorporated an antifungal agent into an adhesive and evaluated the influence on biofilm formation and adhesive strength. Design: Specimens in resin based on polymethylmethacrylate (PMMA) were divided into groups: PMMA (No Adhesive); PMMA+Ultra Corega Cream Adhesive and PMMA+Ultra Corega Cream Adhesive+Nystatin. Biofilm of Candida albicans was grown on the specimens and the cell viability was investigated by counting colony forming units (CFU/mL). Adhesive strength was tested after 5 minutes, 6 and 12 hours. For microbiological analysis, data were evaluated by Kruskal-Wallis and Dunn's post test, and for analysis of adhesive strength by two-way ANOVA and Bonferroni post test (α=.05). Results: There was a reduction in biofilm formation on the surface of the nystatin-modified adhesive (p<.001). After 5 minutes, the adhesive strength of the modified Ultra Corega Cream was greater than that of the commercial product (p=.048), with no significant difference in the other times (p>.05). Implications: The incorporation of nystatin on denture adhesive reduced biofilm accumulation, with a positive influence on the initial adhesive strength, it may be a viable alternative for delivering the drug to the patient.

Referências

Nicolas E, Veyrune J, Lassauzay C. A six-month assessment of oral health-related quality of life of complete denture wearers using denture adhesive: a pilot study. J Prosthodont [Internet]. 2010 [cited 2023 Mar 24];19:443–448. Available from: https://doi.org/10.1111/j.1532-849X.2010.00601.x

Cardoso M, Balducci I, Telles DM, Lourenço EJV, Júnior LN. Edentulism in Brazil: trends, projections and expectations until 2040. Cien. Saude Colet [Internet]. 2016 [cited 2023 Mar 24];21:1239-1246. Available from: https://doi.org/10.1590/1413-81232015214.13672015

Shamsolketabi S, Nili M. The effect of denture adhesive on the efficiency of complete denture in patients with different alveolar ridges. Dent Res J [Internet]. 2018 [cited 2023 Mar 24];15:271-275. Available from: https://pubmed.ncbi.nlm.nih.gov/30123304/

Han JM, Zhang H, Choe HS, Lin H, Zheng G, Hong G. Abrasive wear and surface roughness of contemporary dental composite resin. Dent Mater J [Internet]. 2014 [cited 2023 Mar 24];33:725-732. Available from: https://doi.org/10.4012/dmj.2013-339

Adisman IK. The use of denture adhesives as an aid to denture treatment. J Prosthet Dent [Internet]. 1989 [cited 2023 Mar 24];62:711-715. Available from: https://doi.org/10.1016/0022-3913(89)90598-2

Kore DR, Kattadiyil MT, Hall DB, Bahjiri K. In vitro comparison of the tensile bond strength of denture adhesives on denture bases. J Prosthet Dent [Internet]. 2013 [cited 2023 Mar 24];110:488-493. Available from: https://doi.org/10.1016/j.prosdent.2013.09.014

Papadiochou S, Emmanouil I, Papadiochos I. Denture adhesives: A systematic review. J Prosthet Dent [Internet]. 2015 [cited 2023 Mar 24];113:391-397. Available from: https://doi.org/10.1016/j.prosdent.2014.11.001

Huang F, Wu W. Antidiabetic effect of a new peptide from Squalus mitsukurii liver (S-8300) in alloxan-diabetes. Clin Exp Pharmacol Physiol [Internet]. 2005 [cited 2023 Mar 24];32:521–525. Available from: https://doi.org/10.1211/jpp.57.12.0007

Aslaminehr M, Rezvani S, Mahmoudi A, Moosavi N. Comparison of Candida albicans adherence to conventional acrylic denture base materials and injection molding acrylic materials. J Dent (Shiraz) [Internet]. 2017 [cited 2023 Mar 24];18:61–64. Available from: https://pubmed.ncbi.nlm.nih.gov/28280761/

Coulthwaite L, Verran J. Potential pathogenic aspects of denture plaque. Br J Biomed Sci [Internet]. 2007 [cited 2023 Mar 24];64:180-189. Available from: https://doi.org/10.1080/09674845.2007.11732784

O'donnell LE, Smith K, Williams C, Nile CJ, Lappin DF, Bradshaw D, et al. Dentures are a Reservoir for Respiratory Pathogens. J Prosthodont [Internet]. 2016 [cited 2023 Mar 24];25:99-104. Available from: https://doi.org/10.1111/jopr.12342

Bueno MG, Urban VM, Barbério GS, da Silva WJ, Porto VC, Pinto LR, et al. Effect of antimicrobial agents incorporated into resilient denture relines on the Candida albicans biofilm. Oral Dis [Internet]. 2015 [cited 2023 Mar 24];21:57-65. Available from: https://doi.org/10.1111/odi.12207

Lyu X, Zhao C, Yan ZM, Hua H. Efficacy of nystatin for the treatment of oral candidiasis: a systematic review and meta-analysis. Drug Des Devel Ther [Internet]. 2016 [cited 2023 Mar 24];10:1161-71. Available from: https://doi.org/10.2147/DDDT.S100795

Nomura T, Murakami T, Shimoyama Y, Kobayashi T, Furuya J, Sasaki M, et al. Effects of denture adhesives on growth and morphological transformation of Candida albicans. J Prosthodont Res [Internet]. 2020 [cited 2023 Mar 24];64:78-84. Available from: https://doi.org/10.1016/j.jpor.2019.04.006

Almeida NLM, Saldanha LL, Silva RA, Pinke KH, da Costa EF, Porto VC, et al. Antimicrobial activity of denture adhesive associated with Equisetum giganteum- and Punica granatum-enriched fractions against Candida albicans biofilms on acrylic resin surfaces. Biofouling [Internet]. 2018 [cited 2023 Mar 24];34:62-73. Available from: https://doi.org/10.1080/08927014.2017.1407408

Castro DT, Duarte VBB, Schiavon MA, Reis AC. Effect of Thermomechanical Fatigue on Shear Strength between a Conventional and an Experimental Polymer for Prosthetic Application. J Health Sci [Internet]. 2019 [cited 2023 Mar 24];21:97-102. Available from: https://doi.org/10.17921/2447-8938.2019v21n2p97-102

Salles MM, Oliveira VC, Souza RF, Silva CH, Paranhos HF. Antimicrobial action of sodium hypochlorite and castor oil solutions for denture cleaning - in vitro evaluation. Braz Oral Res [Internet]. 2015 [cited 2023 Mar 24];29:1-6. Available from: https://doi.org/10.1590/1807-3107BOR-2015.vol29.0104

Costa MS, Lovato da Silva CH, Oliveira VC, Watanabe E, dos Reis AC, Lepri CP, et al. Effects of Different Forms of Denture Adhesives on Biofilm Formation, Adhesive Strength and Hygiene of Complete Dentures. Int J Prosthodont [Internet]. 2022 [cited 2023 Mar 24];35:784-792. Available from: https://doi.org/10.11607/ijp.7188

de Oliveira Junior NM, Mendoza Marin DO, Leite ARP, Pero AC, Klein MI, Compagnoni MA. Influence of the use of complete denture adhesives on microbial adhesion and biofilm formation by single- and mixed-species. PLoS One [Internet]. 2018 [cited 2023 Mar 24];13:e0203951. Available from: https://doi.org/10.1371/journal.pone.0203951

Bharti B, Li H, Ren Z, Zhu R, Zhu Z. Recent advances in sterilization and disinfection technology: A review. Chemosphere [Internet]. 2022 [cited 2023 Mar 24];308:136404. Available from: https://doi.org/10.1016/j.chemosphere.2022.136404

Boucenna A, Oturan N, Chabani M, Bouafia-Chergui S, Oturan MA. Degradation of Nystatin in aqueous medium by coupling UV-C irradiation, H2O2 photolysis, and photo-Fenton processes. Environ Sci Pollut Res Int [Internet]. 2019 [cited 2023 Mar 24];26:23149-23161. Available from: https://doi.org/10.1007/s11356-019-05530-2

Cartagena AF, Esmerino LA, Polak-Junior R, Parreiras SO, Michel MD, Farago PV, et al. New denture adhesive containing miconazole nitrate polymeric microparticles: Antifungal, adhesive force and toxicity properties. Dent Mater [Internet]. 2017 [cited 2023 Mar 24];33:e53-e61. Available from: https://doi.org/10.1016/j.dental.2016.09.039

Haraldson T, Karlsson U, Carlsson GE. Bite force and oral function in complete denture. J Oral Rehabil [Internet]. 1979 [cited 2023 Mar 24];1:41-8. Available from: https://doi.org/10.1111/j.1365-2842.1979.tb00403.x

Nishi Y, Nomura T, Murakami M, Kawai Y, Nishimura M, Kondo H, et al. Effect of denture adhesives on oral moisture: A multicenter randomized controlled trial. Prosthodont Res [Internet]. 2020 [cited 2023 Mar 24];64:281–288. Available from: https://doi.org/10.1016/j.jpor.2019.08.004

Nosrathezi T, Nosrathezi M, Nosrathezi S, Fatemeh L. The comparison of the effect of curcumin with nystatin on inhibition level of Candida albicans. J Exp Pharmacol [Internet]. 2019 [cited 2023 Mar 24];11: 93–97. Available from: https://doi.org/10.2147/JEP.S215843

Sklenár Z, Scigel V, Horácková K, Slanar O. Compounded preparations with nystatin for oral and oromucosal administration. Acta Pol Pharm [Internet]. 2013 [cited 2023 Mar 24];70:759-762. Available from: https://pubmed.ncbi.nlm.nih.gov/23923400/

Hotta J, Garlet GP, Cestari TM, Lima JFL, Porto VC, Urban VM, et al. In vivo biocompatibility of an interim denture resilient liner containing antifungal drugs. J Prosthet Dent [Internet]. 2019 [cited 2023 Mar 24];121:135-142. Available from: https://doi.org/10.1016/j.prosdent.2018.02.005

Prasad R, Nair R, Banerjee A. Emerging mechanisms of drug resistance in Candida albicans. Prog Mol Subcell Biol [Internet]. 2019 [cited 2023 Mar 24];58:135–153. Available from: https://doi.org/10.1007/978-3-030-13035-0_6

Fuentefria AM, Pippi B, Dalla Lana DF, Donato KK, de Andrade SF. Antifungals discovery: an insight into new strategies to combat antifungal resistance. Lett Appl Microbiol [Internet]. 2018 [cited 2023 Mar 24];66:2–13. Available from: https://doi.org/10.1111/lam.12820

Kessler SQS, Lang PM, Dal-Pizzol TS, Montagner F. Resistance profiles to antifungal agents in Candida albicans isolated from human oral cavities: systematic review and meta-analysis. Clin Oral Investig [Internet]. 2022 [cited 2023 Mar 24];26:6479-6489. Available from: https://doi.org/10.1007/s00784-022-04716-2

Garaicoa JL, Fischer CL, Bates AM. Promise of combining antifungal agents in denture adhesives to fight candida species infections. J Prosthodont [Internet]. 2018 [cited 2023 Mar 24];27:755–762. Available from: https://doi.org/10.1111/jopr.12565

Schneid TR. An in vitro analysis of a sustained release system for the treatment of denture stomatitis. Spec Care Dentist [Internet]. 1992 [cited 2023 Mar 24];12:245-250. Available from: https://doi.org/10.1111/j.1754-4505.1992.tb00458.x

Rai A, Misra SR, Panda S, Sokolowski G, Mishra L, Das R, Lapinska B. Nystatin Effectiveness in Oral Candidiasis Treatment: A Systematic Review & Meta-Analysis of Clinical Trials. Life (Basel) [Internet]. 2020 [cited 2023 Mar 24];12:1677. Available from: https://doi.org/10.3390/life12111677

Alcântara CS, Macêdo AFC, Gurgel BCV, Jorge JH, Neppelenbroek KH, Urban VM. Peel bond strength of resilient liner modified by the addition of antimicrobial agents to denture base acrylic resin. J Appl. Oral Sci [Internet]. 2012 [cited 2023 Mar 24];20:607-612. Available from: https://doi.org/10.1590/s1678-77572012000600004

Hebeka EK, Solotorovsky MJ. Development of resistance to polyene antibiotics in Candida albicans. Bacteriol [Internet]. 1965 [cited 2023 Mar 24];89:1533-9. Available from: https://doi.org/10.1128/jb.89.6.1533-1539.1965

Urban VM, de Souza RF, Arrais CA, Borsato KT, Vaz LG. Effect of the association of nystatin with a tissue conditioner on its ultimate tensile strength. J Prosthodont [Internet]. 2006 [cited 2023 Mar 24];15(5):295-299. Available from: https://doi.org/10.1111/j.1532-849X.2006.00130.x

An Y, Li D, Roohpour N, Gautrot JE, Barber AH. Failure mechanisms in denture adhesives. Dent Mater [Internet]. 2016 [cited 2023 Mar 24];32:615-623. Available from: https://doi.org/10.1016/j.dental.2016.01.007

Zhao K, Cheng XR, Chao YL, Li ZA, Han GL. Laboratory evaluation of a new denture adhesive. Dent Mater [Internet]. 2004 [cited 2023 Mar 24];20:419-424. Available from: https://doi.org/10.1016/j.dental.2002.12.001

Zissis AJ, Polyzois GL, Yannikakis SA, Harrison A. Roughness of denture materials: a comparative study. Int J Prosthodont [Internet]. 2000 [cited 2023 Mar 24];13:136-140. Available from: https://pubmed.ncbi.nlm.nih.gov/11203622/

Downloads

Publicado

2024-03-18

Como Citar

Alvim , G. C. ., Beatriz da Silva , C. B. da ., Oliveira, V. de C., Reis, A. C. dos ., Watanabe, E. ., Penazzo Lepri, C. P., & Castro, D. T. de . (2024). Evaluation of the antifungal effect and adhesive strength of a denture adhesive supplemented with nystatin. Revista Prevenção De Infecção E Saúde, 9(1). https://doi.org/10.26694/repis.v9i1.4337

Edição

v. 9 n. 1 (2023): Revista Prevenção de Infecção e Saúde

Seção

Artigo Original

Artigos mais lidos pelo mesmo(s) autor(es)